Figure 6. Immunohistochemistry for cleaved caspase-3 following I/R.

(A) Representative images of caspase-3 staining in kidney sections from WT sham, ACE2 sham, WT IR and ACE2 IR mice; magnification: 200x. Positively staining cells were counted in randomly chosen areas, and the number of positive cells and size of each area were recorded. (B) Quantitation of caspase-3 staining by numbers of positive cells per mm² of tissue. Results are presented as mean ± SE. n = 8 for WT sham; n = 7 for ACE2 sham; n = 12 for WT IR; n = 10 for ACE2 IR. * p<0.05 vs. WT sham. ^† p<0.05 vs. WT IR.