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. 2013 Aug 9;8(8):e70987. doi: 10.1371/journal.pone.0070987

Figure 3. MiR-185 and 342 induce caspase-dependent apoptotic death.

Figure 3

A, An Annexin V-FITC/PI staining apoptotic assay and flow cytometry were performed in LNCaP and C4-2B cells transfected with miRNAs. Both miR-185 and 342 increased the apoptotic cell fractions (both early and late apoptotic cell fractions; P<0.005) compared with the control groups. B, Caspase 3/7 activities were significantly increased by miR-185 and 342 in LNCaP and C4-2B cells. Caspase 3/7 activities (fold) were assigned as 1.0 in non-transfected (−) cells. **, P < 0.005 significant differences from NC. Data represent the mean ± SD of two independent triplicate experiments. C, MiR-185 and 342 decreased pro-caspase 9, 3 and PARP, and activated cleaved caspase 3 and PARP expression in LNCaP cells as assayed by Western blot. β2M was used as a loading control. C: cleaved.