Figure 1. (A) Regulation of Net1A localization by integrin ligation. Integrin binding to the ECM promotes recruitment of the FAK/DOCK180 signaling complex, which stimulates Rac1 activation. Rac1 then signals to Net1A in the nucleus, causing Net1A export to the plasma membrane where it activates RhoA. Active RhoA promotes bundling of actin stress fibers, actomyosin contraction, and focal adhesion maturation. After Rac1 activation ceases Net1A is removed from the extranuclear space by proteasome-mediated degradation. (B) Subcellular distribution of Net1A during cell spreading. Shown are MCF7 cells that have been transfected with HA-Net1A, serum starved overnight, and then left adherent or replated on collagen IV for 30 or 90 min. After fixation the cells were stained for HA-Net1A (green), F-actin (red), and DNA (blue).