Table 1. Primers and annealing temperatures (temp) for Notch and VEGF pathway molecules.

Genes	Forward (5′to 3′)	Reverse (5′to 3′)	Size(bp)	temp(°C)
Notch1	TCAGCGGGATCCACTGTGAG	ACACAGGCAGGTGAACGAGTTG	104	62
DLL4	CCCTGGCAATGTACTTGTGAT	TGGTGGGTGCAGTAGTTGAG	74	58
VEGF	CCTGGTGGACATCTTCCAGGAGTACC	GAAGCTCATCTCTCCTATGTGCTGGC	196	61
VEGFR1	CTGGACTGACAGCAAACCCAAG	CCACAGCTGGAATGGCAGAA	117	60
VEGFR2	AGCCAGCTCTGGATTTGTGGA	CATGCCCTTAGCCACTTGGAA	133	58
HIF-1a	CATCAGCTATTTGCGTGTGAGGA	AGCAATTCATCTGTGCTTTCATGTC	83	58
GAPDH	GCACCGTCAAGGCTGAGAAC	TGGTGAAGACGCCAGTGGA	138	62