Skip to main content
. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Prostaglandins Other Lipid Mediat. 2013 Feb 27;0:31–41. doi: 10.1016/j.prostaglandins.2013.01.005

Fig. 3.

Fig. 3

Effects of 5,14-HEDGE and 20-HEDE on changes in renal MEK1 activity measured 4 h after saline (vehicle) (4 ml/kg, i.p.) or LPS (10 mg/kg, i.p.) injection to conscious rats. 5,14-HEDGE (30 mg/kg, s.c.) and/or 20-HEDE (30 mg/kg, s.c.) were given 1 h after administration of saline or LPS. MEK1 protein expression and p-MEK1 (Ser217/221) protein levels in tissue homogenates were measured by immunoblotting and ELISA Kit, respectively. MEK1 activity was calculated as the ratio of p-MEK1 (Ser217/221)/MEK1 protein levels. Data are expressed as means ± S.E.M of 4–7 animals. *Significant difference from the corresponding value seen in rats treated with saline (p < 0.05). #Significant difference from the corresponding value seen in the rats treated with LPS (p < 0.05). §Significant difference from the corresponding value seen in the rats treated with LPS and 5,14-HEDGE (p < 0.05).