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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Prostaglandins Other Lipid Mediat. 2013 Feb 27;0:31–41. doi: 10.1016/j.prostaglandins.2013.01.005

Fig. 4.

Fig. 4

Effects of 5,14-HEDGE and 20-HEDE on changes in renal ERK1/2 activity measured 4 h after saline (vehicle) (4 ml/kg, i.p.) or LPS (10 mg/kg, i.p.) injection to conscious rats. 5,14-HEDGE (30 mg/kg, s.c.) and/or 20-HEDE (30 mg/kg, s.c.) were given 1 h after administration of saline or LPS. ERK1/2 protein expression and p-ERK1/2 (pTpY185/187) protein levels in tissue homogenates were measured by immunoblotting and ELISA Kit, respectively. ERK1/2 activity was calculated as the ratio of p-ERK1/2 (pTpY185/187)/ERK1/2 protein levels. Data are expressed as means ± S.E.M of 4–10 animals. *Significant difference from the corresponding value seen in rats treated with saline (p < 0.05). #Significant difference from the corresponding value seen in the rats treated with LPS (p < 0.05). §Significant difference from the corresponding value seen in the rats treated with LPS and 5,14-HEDGE (p < 0.05).