Fig. 5.
Effects of 5,14-HEDGE and 20-HEDE on changes in renal IκB-α protein expression and phosphorylation measured 4 h after saline (vehicle) (4 ml/kg, i.p.) or LPS (10 mg/kg, i.p.) injection to conscious rats. 5,14-HEDGE (30 mg/kg, s.c.) and/or 20-HEDE (30 mg/kg, s.c.) were given 1 h after administration of saline or LPS. IκB-α and p-IκB-α (Ser32) protein levels in tissue homogenates were measured by immunoblotting. Data are expressed as means ± S.E.M of 4 animals. *Significant difference from the corresponding value seen in rats treated with saline (p < 0.05). #Significant difference from the corresponding value seen in the rats treated with LPS (p < 0.05).