Table 1.
Specification of immunoreagents
| Antigen | Code | Host species | Dilution | Supplier |
|---|---|---|---|---|
| Primary antibodies | ||||
| CB | CB-38 | Rabbit | 1:20,000 | SWANT |
| TH | MAB 318 | Mouse | 1:80 | Chemicon |
| NPY | NZ1115 | Rat | 1:300 | Biomol |
| SOM | 8330-0009 | Rat | 1:100 | Biogenesis |
| VIP | 9535-0504 | Mouse | 1:2,000 | Biogenesis |
| NOS | N2280 | Mouse | 1:2,000 | Sigma |
| LENK | 4140-0355 | Mouse | 1:1,000 | Biogenesis |
| SP | 8450-0505 | Rat | 1:300 | Biogenesis |
| VAChT | H-V007 | Goat | 1:2,000 | Phoenix |
| GAL | T-5036 | Guinea pig | 1:1,000 | Peninsula |
| Secondary reagents | ||||
| Donkey anti-mouse IgG (H+L) conjugated with FITC | 1:800 | Jackson | ||
| Donkey anti-rat IgG (H+L) conjugated with FITC | 1:800 | Jackson | ||
| Donkey anti-guinea pig IgG (H+L) conjugated with FITC | 1:1,000 | Jackson | ||
| Donkey anti-goat IgG (H+L) conjugated with FITC | 1:1,000 | Jackson | ||
| Biotinylated goat antirabbit immunoglobulins | 1:1,000 | DAKO | ||
| Biotin conjugated F(ab) fragment anti-rabbit IgG | 1:1,000 | BioTrend | ||
| CY3-conjugated streptavidin | 1:9,000 | Jackson | ||