Figure 5.

Sensitivity of the mekk1/2/3 mutant to other amino acids.

(a) Seedlings (5 days old) of Col–8 and mekk1/2/3 were transferred to plates containing either 0.5 mm Gln alone (control) or 0.5 mm Gln plus 0.5 mm l–Glu, 2 mm Gly, 0.5 mm Cys, 1 mm Ser or 2 mm Asn. The percentage inhibition of primary root growth by each amino acid was determined by measuring primary root growth over the following 8 days and comparing it with the control. Asterisks indicate where the difference between the parental line and the mutant is statistically significant (Student's t–test: *P < 0.05; ***P < 0.001).

(b) Time course of primary root growth of Col–8 and mekk1/2/3 seedlings after transfer to control plates or plates containing 1 μm flg22.