Fig. 6.

Natural competence in the presence and absence of AMP. sBHI-grown cells were transferred to MIV with (grey bars) or without (black bars) 1 mM AMP (A), or with increasing concentrations of AMP (C) for 100 min before measuring transformation frequency. Each bar or point represents the mean of three biological replicates ± standard deviations. Circles: WT (wild type), squares: ΔpurH, triangles: sxy-1.

B. qPCR (top) and western blot (bottom) analysis of sxy RNA and protein expression in the presence (grey bars) and absence (black bars) of 1 mM AMP after 100 min in MIV (see Experimental procedures for details). Error bars represent the mean of three biological replicates. Sxy protein levels are relative to the unidentified protein used for internal standardization in Cameron et al. (2008). A representative Western blot below the bottom graph shows Sxy protein and the standardization protein.