Fig. 4.

Transcript level changes upon MG stress are similar between strains MG1655 and MJF632, and correlate with changes in RNAP occupancy. Transcript levels for a number of genes in strain MG1655 (black bars) and MJF632 (ΔkefGB, ΔkefFC; gray bars) were determined by qRT-PCR. Cells were grown and treated exactly as in Type I ChIP-chip experiments. Transcript levels were normalized against the internal control genes topB, trkA and polA. Changes in transcript levels are expressed as fold-changes relative to untreated control samples. Error bars indicate the standard deviation of three independent experiments.