TABLE 2.
Quantitation of Sema-2b Loss-of-Function Phenotypes
| Genotype | Genetic description | ISNb bypass(na) | E.V.Pc/Embryo(Nb) |
|---|---|---|---|
| F02042/f02042 | sema2b transposon mutant | 9.2% (1313) | 0.83 (157) |
| Df7142/Df7142 | sema2b deficiency mutant | 8.6% (421) | 0.87 (39) |
| D05894/d05894 | sema2b isogenic wild-type | 0.9% (423) | ND |
| W1118 | Wild-type | 1.5%(334) | ND |
| Ex9/f02042 | Precise Excision Het. | 0.7% (430) | 0.05 (38) |
| ExMN3/f02042 | Precise Excision Het. | 2.3% (397) | 0.08 (36) |
| Ex9/Ex9 | Precise Excision Hom. | 1.6% (312) | 0.14 (28) |
| ExMN3/ExMN3 | Precise Excision Hom. | 0.4% (446) | 0.03 (39) |
a
n is the total number of segments scored. N is the number of embryos scored.
E.V.P. refers to Ectopic Ventral Projections, which were scored as 1D4-positive. Axons were detected ventral to their normal location. ISNb bypass phenotypes were scored as any lack of innervation from the ISNb nerve that was accompanied by a concomitant increase in ectopic projections from the ISN. Het., heterozygotes; Hom., homozygotes.