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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Cell Biochem. 2013 Jun;114(6):1414–1423. doi: 10.1002/jcb.24483

Fig. 5. Effect of R14-RBD/GPSM on RGS protein GAP activity.

Fig. 5

Membranes derived from Sf9 cells co-expressing the M2 muscarinic acetylcholine receptor plus heterotrimeric Gi1, Gi2, Gi3 or Go were assayed at 30°C for 5 minutes with the agonist carbachol (100 μM) either alone or in the presence of either R14-RBD/GPSM (1 μM), RGS4 (300 nM) or both . Nonspecific signal with each membrane was defined as that observed in the absence of RGS protein and in the presence of tropicamide (10 μM) and this was subtracted to yield the values indicated. Data represent mean values ± SEM of at least three independent experiments. *, p<0.05, **, p<0.01, ***, p<0.001, compared with agonist alone, (one-way ANOVA with Tukey's multiple comparison test).