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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Cell Biochem. 2013 Jun;114(6):1414–1423. doi: 10.1002/jcb.24483

Fig. 6. The GPSM domain of RGS14 does not inhibit receptor-stimulated GTPase activity.

Fig. 6

Membranes derived from Sf9 cells co-expressing the M2 muscarinic acetylcholine receptor plus heterotrimeric Gi3 were assayed at 30°C for 5 minutes with the agonist carbachol (100 μM) either alone or in the presence of RGS4 (300nM) and different mutants of RGS14 (1 μM). Nonspecific signal with each membrane was defined as that observed in the absence of RGS protein and in the presence of the antagonist tropicamide (10 μM) and this was subtracted to yield the values indicated. Data represent mean values ± SEM of at least three independent experiments. **, p <0.01, compared with agonist in the presence of RGS4, #, p<0.01compared with agonist in the presence of RGS4 and R14-RBD/GPSM, (one-way ANOVA with Tukey's multiple comparison test).