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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Lung Cancer. 2013 May 16;81(2):167–173. doi: 10.1016/j.lungcan.2013.04.017

Fig. 5.

Fig. 5

Activation of p53 with Nutlin-3a enhances IR-induced tumor cell killing in NSCLC cells. (A) Lung cancer cells were pre-incubated with 3 μM of Nutlin-3a (Nut) for 30min prior to irradiation. The status of p53 activation was determined by p-p53 (Ser15) Western blotting analyses at 24 h after 4Gy of IR. The expression of total p53 and p21 were also determined by Western blotting. (B) Clonogenic assay was employed to detect IR-induced tumor cell killing. (C) SA-β-gal staining was performed to determine senescent cells in irradiated H460 cells. (D) BrdU incorporation assay was used to detect the proliferating cells. a, p < 0.05 vs. control (Ctl); b, p < 0.001 vs. Ctl; c, p < 0.01 vs. IR.