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ACD of T cells requires contact with the antigen presenting cell. The ratio of proximal to distal polarization was assessed as in Fig 2 for aPKC (24 cells), Scribble (14 cells), PAR-3 (8 cells) and Numb (10 cells) in mitotic cells unattached to a DC (representative images below) compared to mitotic cells attached to a DC. Scale bars; 10 µm.
