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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Neurotox Res. 2013 Apr 9;24(4):472–477. doi: 10.1007/s12640-013-9390-7

Figure 2.

Figure 2

Effect of hypoxic preconditioning on ethanol-induced cell death. SH-SY5Y cells were cultured and exposed to hypoxic or controlled environment (normoxia) for 8 hours as described in Fig. 1. After that, cells were treated with ethanol (0.4% or 316 mg/dl) for 12–48 hours. Cell viability was determined by MTT assay as described under the Materials and Methods. Ct: normoxia; Hypoxia: 1% oxygen; EtOH: 0.4% (316 mg/dl) ethanol; H+E: hypoxia plus ethanol. The experiment was replicated three times. * denotes a statistically significant difference from no-ethanol-treated controls. # denotes a significant difference from ethanol-treated cells under normoxia condition.