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Manipulation of hypoxia-induced autophagy. SH-SY5Y cells were exposed to a hypoxic or controlled environment in the presence or absence of rapamycin (Rap, 10 μM) or bafilomycin (Baf, 10 nM) for 8 hours. The expression of LC3 was analyzed by immunoblotting as described in Fig. 1. C: control; H: hypoxia.
