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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Mol Reprod Dev. 2013 Apr 4;80(8):10.1002/mrd.22168. doi: 10.1002/mrd.22168

Table 2.

In vitro culture conditions for different murine stem cell populations

ESC EpiSC EG cells MAPC VSEL
Tissue of Origin Blastocyst: Inner cell mass Epiblast, 5.5dpc PGC, 8.5 dpc, base of allantois Adult bone marrow, plastic adherent Adult bone marrow, Lin− Sca-1+ CD45−
Culture conditions/Cytokines LIF (BMP4) Activin A, bFGF LIF, bFGF, mSCF (BMP4) LIF, EGF, PDGFbb, 5%O2 None defined
Culture Conditions/Feeder Cells MEF sl4-m220 cells C2C12 Self renewal not demonstrated

ESC = Embryonic Stem Cells, EpiSC = Epiblast stem cells, EG cells = Embryonic germ cells, MAPC = Multipotent adult ptogenitor cells, VSEL= very small embryonic like cells. LIF: Leukemia inhibitory factor. MEF: Murine Embryonic Fibroblasts. D.p.c.: day post coitum. (BMP4): BMP4 is secreted by feeder cells, not added to culture media. References: Tremml et al 2008, Tesar et al. 2007, Brons et al. 2007, Jiang et al. 2002; Matsui et al. 1992, Kucia 2008a.