Table 2.
ESC | EpiSC | EG cells | MAPC | VSEL | |
---|---|---|---|---|---|
Tissue of Origin | Blastocyst: Inner cell mass | Epiblast, 5.5dpc | PGC, 8.5 dpc, base of allantois | Adult bone marrow, plastic adherent | Adult bone marrow, Lin− Sca-1+ CD45− |
Culture conditions/Cytokines | LIF (BMP4) | Activin A, bFGF | LIF, bFGF, mSCF (BMP4) | LIF, EGF, PDGFbb, 5%O2 | None defined |
Culture Conditions/Feeder Cells | MEF | sl4-m220 cells | C2C12 Self renewal not demonstrated |
ESC = Embryonic Stem Cells, EpiSC = Epiblast stem cells, EG cells = Embryonic germ cells, MAPC = Multipotent adult ptogenitor cells, VSEL= very small embryonic like cells. LIF: Leukemia inhibitory factor. MEF: Murine Embryonic Fibroblasts. D.p.c.: day post coitum. (BMP4): BMP4 is secreted by feeder cells, not added to culture media. References: Tremml et al 2008, Tesar et al. 2007, Brons et al. 2007, Jiang et al. 2002; Matsui et al. 1992, Kucia 2008a.