Table 3.
Culture Condition | Result |
---|---|
SCF, LIF, bFGF, BMP4, SDF1, Forskolin, RA on fibronectin and laminin | Larger cells, Vasa positive, no proliferation |
LIF, Activin A, bFGF, SCF, EGF on fibronectin and laminin | Negative |
PDGFbb, LIF, EGF, Dexamethasone1 | Negative |
PDGFbb, LIF, EGF, Dexamethasone + anti TGFbeta1 | Negative |
PDGFbb, LIF, EGF, Dexamethasone, IGF21 | Negative |
PDGFbb, LIF, EGF, Dexamethasone, Activin A1 | Negative |
PDGFbb, LIF, EGF, Dexamethasone, IGF2, Activin A1 | Negative |
PDGFbb, LIF, EGF, Dexamethasone, IGF2, Activin A, IL-6, bFGF1 | Negative |
IGF2, IL-6, Activin A, PDGFbb2 | Negative |
IGF2, IGF1, Activin A, PDGFbb2 | Negative |
IGF2, IGF1, LIF, PDGFbb2 | Negative |
IGF2, IGF1, LIF, PDGFbb, Activin A, LIF, EGF, FGF2 | Negative |
IGF1,b FGF, EGF3 | Negative |
IGF1, bFGF, EGF, LIF3 | Negative |
bFGF, EGF, LIF3 | Negative |
bFGF, Activin A3 | Negative |
Transwell over C2C12 cell, serum free | Spheres affer 3 weeks – Oct4 not detected by PCR |
Negative = No Proliferation, cells gradually lost. Culture medium: DMEM/F12 + B27+N2 supplements. Density: 1000 cells/100µl/well.
2–10% knockout serum replacement. Tested both fibronectin or uncoated plastic, hyopxia and normoxia
tested both laminin or fibronectin
Tested both hypoxia or normoxia, hydrogel or laminin.