Table 3.
Conditions tested for Culture of VSEL’s
| Culture Condition | Result |
|---|---|
| SCF, LIF, bFGF, BMP4, SDF1, Forskolin, RA on fibronectin and laminin | Larger cells, Vasa positive, no proliferation |
| LIF, Activin A, bFGF, SCF, EGF on fibronectin and laminin | Negative |
| PDGFbb, LIF, EGF, Dexamethasone1 | Negative |
| PDGFbb, LIF, EGF, Dexamethasone + anti TGFbeta1 | Negative |
| PDGFbb, LIF, EGF, Dexamethasone, IGF21 | Negative |
| PDGFbb, LIF, EGF, Dexamethasone, Activin A1 | Negative |
| PDGFbb, LIF, EGF, Dexamethasone, IGF2, Activin A1 | Negative |
| PDGFbb, LIF, EGF, Dexamethasone, IGF2, Activin A, IL-6, bFGF1 | Negative |
| IGF2, IL-6, Activin A, PDGFbb2 | Negative |
| IGF2, IGF1, Activin A, PDGFbb2 | Negative |
| IGF2, IGF1, LIF, PDGFbb2 | Negative |
| IGF2, IGF1, LIF, PDGFbb, Activin A, LIF, EGF, FGF2 | Negative |
| IGF1,b FGF, EGF3 | Negative |
| IGF1, bFGF, EGF, LIF3 | Negative |
| bFGF, EGF, LIF3 | Negative |
| bFGF, Activin A3 | Negative |
| Transwell over C2C12 cell, serum free | Spheres affer 3 weeks – Oct4 not detected by PCR |
Negative = No Proliferation, cells gradually lost. Culture medium: DMEM/F12 + B27+N2 supplements. Density: 1000 cells/100µl/well.
1
2–10% knockout serum replacement. Tested both fibronectin or uncoated plastic, hyopxia and normoxia
2
tested both laminin or fibronectin
3
Tested both hypoxia or normoxia, hydrogel or laminin.