Skip to main content
. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: J Neuroimmune Pharmacol. 2013 May 5;8(4):975–987. doi: 10.1007/s11481-013-9464-6

Figure 4.

Figure 4

Effects of Tat on kinetic analysis of [3H]DA uptake in WT hDAT and mutant. (A) CHO cells transfected with WT or Y470H-hDAT were preincubated with or without released Tat1-72 (1.0 ng/ml) at room temperature for 20 min followed by the addition of one of six mixed concentrations of the [3H]DA. In parallel, nonspecific uptake at each concentration of [3H]DA (in the presence of 10 μM nomifensine, final concentration) was subtracted from total uptake to calculate DAT-mediated uptake. (B) [3H]DA uptake in cells transfected with WT or Y470H-hDAT was determined in the presence or absence of recombinant Tat1-86 (rTat1-86, 350 nM, final concentration). (C) [3H]DA uptake in cells transfected with WT (0.8 μg plasmid cDNA) or Y470H-hDAT (2.4 μg plasmid cDNA) was determined in the presence or absence of rTat1-86 (350 nM). Data are expressed as means from five independent experiments ± S.E.M. *p < 0.05 compared with the respective control values. # p < 0.05 compared to WT hDAT. (n = 5)