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. Author manuscript; available in PMC: 2013 Aug 11.
Published in final edited form as: Nat Struct Mol Biol. 2013 Mar;20(3):267–273. doi: 10.1038/nsmb.2506

Figure 3.

Figure 3

Determinants of nucleosome positioning. (a) Nucleosome-depleted regions (NDRs) are generated either by poly (dA:dT) tracts and/or by transcription factors and their recruited nucleosome remodeling complexes. Gray circles indicate nucleosomes. (b) Nucleosomes located at highly preferred positions (black circles) flanking the NDR are generated by nucleosome-remodeling complexes (for example, Isw2 and RSC, likely in a transcription-independent manner), and fine-tuned by the Pol II preinitiation complex (PIC) and associated factors. (c) Positioning of the more downstream nucleosomes depends on transcriptional elongation, and the recruitment of nucleosome-remodeling activities (for example, Chd1 and Isw1) and histone chaperones by the elongating Pol II machinery. This figure, which was modified from ref. 36, does not include DNA sequence determinants of rotational positioning (Fig. 2) or contributions to nucleosome spacing by histone H1.