Fig. 1.

Transgenic mice synthesizing AcGFP driven by a 2.7-kb region of the Tspo promoter and genotyping strategy. A Scanning image of AcGFP in green, glowing 6-day-old mouse pups from two heterozygous parents viewed by fluorescence imaging as described under Materials and Methods. B Genomic mapping of the Tspo promoter-AcGFP transgenic cassette on chromosome 16. The primers used for genotyping are indicated: primers a/b generate a PCR product that indicates that the mouse lacks the transgene and primers a/c generate a PCR product that indicates that the mouse possesses the transgene. C Genotyping of transgenic mice using PCR with the three primers as indicated in B. One litter of mice generated by heterozygous parents was genotyped as shown here. M, 1kb DNA ladder; +, positive allele with the insertion of Tspo-AcGFP cassette; −, wild-type allele