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. Author manuscript; available in PMC: 2013 Aug 11.
Published in final edited form as: Biol Psychiatry. 2011 Jan 20;70(5):441–448. doi: 10.1016/j.biopsych.2010.11.029

Figure 1.

Figure 1

Time line of the traumatic experience with reminders of stress protocol. Mice undergo baseline acoustic startle response (ASR) testing and shock and no-shock groups are balanced so the average baseline ASR of the two groups does not differ. The following day, mice are exposed to the shock box. On the fourth day after the shock and every following fourth day for a total of six exposures, mice were exposed to the situational reminder, during which each mouse was restricted to the light chamber of the box for 1 minute. The day after the last situational reminder or 25 days after the shock, mice were subjected to a second ASR test (posttest 1). For about a month after posttest 1, mice in behavioral experiments underwent behavioral testing. In c-Fos experiments, mice were euthanized 3 days after ASR posttest 1. To demonstrate persistence of behavioral changes, some mice underwent a third ASR test (posttest2) 56 days or 2 months after the shock. SR, situational reminder.