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. Author manuscript; available in PMC: 2014 May 17.
Published in final edited form as: Mol Biochem Parasitol. 2013 May 17;189(0):43–53. doi: 10.1016/j.molbiopara.2013.05.004

Figure 5. Colocalization of the AKMT truncations and T. gondii α1- tubulin.

Figure 5

A) Intracellular Δakmt parasites co-expressing mTAG-RFP-TgTUBA1 with eGFP tagged SET-cys, Nt-SET-cys, SET-cys-Ct, or full-length. To facilitate the line-scan analyses, maximum intensity projections of deconvolved 3-D stacks are used. Red: mTAG-RFP-TgTUBA1; Green: eGFP fluorescence. Insets: 2X.

B) Structured illumination-based super-resolution imaging of dividing intracellular parasites co-expressing mCherryFP-TgTUBA1 and eGFP-AKMT-full length in the Δakmt background. Arrowheads: daughters. Arrows: daughter cortical microtubules. Contrast of the mCherryFP-TgTUBA1 image is adjusted to display the daughter cortical microtubules. Insets: 2X. Line-scans represent fluorescence intensity variation along the region indicated by the white dotted lines. Green:AKMT. Red:TUBA1. X-axis: distance in pixels. Y-axis: relative intensity in arbitrary units.