Fig. 4.
(a) ATR FT-IR absorbance image at 1656 cm−1 (amide I) of NDF stimulated with TGF-β1 for 24 h. MNF transform was applied to reduce noise and better segregate nuclear from cytoplasmic spectra for subsequent analysis. (b) NDF co-cultured with MCF-7 show intracellular stress fibers in ATR FT-IR absorbance image at 1656 cm−1 after application of MNF transform and limiting image reconstruction to bands that show stress fibers for visualization only. Scale bar is 50 μm.