Fig.7.

Binding of biotinylated JAM-B–EGFP and JAM-C–EGFP chimeras to CHO cells expressing JAM-B and JAM-C. (A) Western blot of biotinylated JAM-C or JAM-B probed with streptavidin–alkaline phosphatase shows the presence of a single species for each protein at the expected molecular weight. (B) Wild-type CHO cells (black line) or CHO cells expressing JAM-B (red and green lines) were incubated with either biotinylated JAM-C–EGFP (black and green lines) or biotinylated JAM-B–EGFP (red line) at 1 μg/ml. Binding was detected with streptavidin–APC, and cells were analyzed by flow cytometry. (C) Wild-type CHO cells (black line) or CHO cells expressing JAM-C (red and green lines) were incubated with either biotinylated JAM-B–EGFP (black and green lines) or biotinylated JAM-C–EGFP (red line). Binding was detected with streptavidin–APC, and cells were analyzed by flow cytometry. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)