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. 2013 Jun 3;2(6):e49. doi: 10.1038/oncsis.2013.14

Figure 2.

Figure 2

AC activates Akt. PPC1 and DU145 prostate cancer cells stably expressing (a) AC or control (PPC1: AC-V5 or LacZ-V5; DU145-AC-EGFP or EGFP), or (b) ASAH1 antisense or empty vector (shAC or pLKO.1) were analyzed by western blotting. (c) MIA, Panc01, SCC14A and PPC1 cells were infected with Ad-AC or Ad-GFP. After 48 h of infection, cells were analyzed by western blotting. (d) DU145 cells were infected with Ad-GFP, Ad-AC or Ad-shAC. After 48 h of infection, cells were analyzed by western blotting. (e) PPC1 cells were infected with Ad-AC or Ad-GFP. After 48 h of infection, cells were analyzed by western blotting. pAkt/tAkt is the ratio of phosphorylated Akt to total Akt normalized to the reference (that is, Ad-GFP is the reference of Ad-AC).