Figure 6.

Knockdown of Erbin promotes proliferation and metastasis of cervical cancer cells in nude mice. (a, b) In all, 5 × 10⁶ HeLa/NC and HeLa/Erbin-sh cells were subcutaneously injected into the right flanks of six-to-seven-week old female Balb/C athymic nude mice. The mice were monitored daily, and tumor volumes estimated according to the formula volume=length × width²/2. At the end of the experiments, the mice were killed. The primary tumors were dissected and weighted. (c, d) HeLa/NC and HeLa/Erbin-sh cells were labeled with CFSE and then implanted into the peritoneal cavity of nude mice. At 72 h after implantation, the apoptotic rates of the tumor cells from peritoneal cavity were determined by FACS analysis of CFSE and Annexin V-phycoerythrin double-positive cells. (e) In all, 5 × 10⁶ HeLa/NC and HeLa/Erbin-sh cells were implanted into the peritoneal cavity of nude mice. After implantation for 56 days, the peritoneal cavities were dissected and photographed. (f) The expression of Erbin in human cervical cancer tissues was analyzed by immunohistochemistry with the antibody against Erbin using a tissue microarray containing cervical cancer and normal cervical tissues.