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. Author manuscript; available in PMC: 2013 Aug 12.
Published in final edited form as: Cancer Res. 2009 Mar 3;69(6):2615–2622. doi: 10.1158/0008-5472.CAN-08-3022

Figure 1.

Figure 1

NKX3.1 up regulates the expression of IGFBP-3 in prostate cancer cell lines.

A, Western blot analysis of cell extracts from stably expressing PC-3(pcDNA3.1) and PC-3(NKX3.1)-1 cell clones that have been analyzed by expression array. B, On the left is western blot analysis of extracts from additional PC-3 clones expressing NKX3.1. One the right is RT-PCR analysis of PC-3(pcDNA3.1) and PC-3(NKX3.1) clones for NKX3.1 and IGFBP-3 expression. A172 cell extract is a positive control for IGFBP-3 expression and LNCaP cell extract is used as a positive control for NKX3.1 expression. C, On the left is western blot analysis of extracts from PC-3 cells transiently transfected with an NKX3.1 expression vector. On the right is western blot analysis of extracts from LNCaP cells transiently transfected with an NKX3.1 expression vector or serum starved in 5% CCS supplemented media overnight and treated with 10nM R1881. D, RT-PCR analysis of IGFBP-4 mRNA expression in PC-3(pcDNA3.1) and PC-3(NKX3.1) clones.