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. Author manuscript; available in PMC: 2013 Aug 12.
Published in final edited form as: Cancer Res. 2009 Mar 3;69(6):2615–2622. doi: 10.1158/0008-5472.CAN-08-3022

Figure 4.

Figure 4

NKX3.1 attenuates IGF-1R downstream signaling.

A, Western blot analysis of extracts from PC-3(pcDNA3.1), PC-3(NKX3.1)-1, and PC-3(NKX3.1)-8 clones serum starved for 16 hours and treated with 100pM IGF-I for 3 minutes. The histogram of PI-3K activation is based upon three separate experiments. Statistical comparisons are indicated by asterisks. Comparisons are versus PC-3(pcDNA3.1) treated with IGF-I. B, Western blot analysis of cell extracts from PC-3(pcDNA3.1) and PC-3(NKX3.1)-1 cells grown in media containing 10% FBS. C, Western blot analysis of cell extracts of PC-3 cells transiently transfected with either a NKX3.1 or a PTEN expression vector.