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. Author manuscript; available in PMC: 2014 Jul 3.

Published in final edited form as: J Am Chem Soc. 2013 Jun 20;135(26):9760–9767. doi: 10.1021/ja401692t

Table 3.

Catalytic Activity of VH1 toward pY Peptides

Peptide No	Peptide Sequence^a	k_cat/K_M (M⁻¹ s⁻¹)
17	EDAFSpYAA	3100 ± 400
18	ADTDFpYAA	2600 ± 100
19	AQDFDpYAA	1600 ± 200
20	FVTDDpYAA	900 ± 200
21	AFGDSpYAA	300 ± 100
22	YSFDPpYAA	3300 ± 100
23	DTADVpYAA	1200 ± 100
24	AApYQWDSF	16000 ± 1000
25	AApYIDWTG	4000 ± 100
26	YDEDFpYDYEF	230000 ± 4000
27	YDEDFpYAYEF	120000 ± 2000
28	YDEDFpYDAEF	24000 ± 1000
29	YDEDFpYDYAF	120000 ± 2000
30	YDEDFpYDYEA	260000 ± 7000
31	YRKRFpYRYKF	500 ± 100

^a

All peptides contained an acetyl group at the N-terminus and a C-terminal amide.