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. Author manuscript; available in PMC: 2013 Aug 12.
Published in final edited form as: Transplantation. 2010 Sep 15;90(5):494–501. doi: 10.1097/TP.0b013e3181c6ff73

FIGURE 3.

FIGURE 3

Complement-mediated killing of swine leukocyte antigen dd (SLAdd) peripheral blood mononuclear cells (PBMC) by sera of all animals that had detectable levels of SLAdd IgG. Negative controls consisted of media alone, treatment of SLAcc PBMC with all serum samples, assessment of preinjection sera (data not shown), and fetal porcine serum (FPS) treatment of SLAdd PBMC (FPS). Positive control consisted of SLAdd PBMC killing by serum from a previously SLAdd-immunized animal (anti-DD). Cytotoxic antibody was detected in sera of all animals that had detectable levels of IgG directed at SLAdd PBMC. (B) Animal 17471 demonstrated numerous pulmonary abscesses, indicating consolidated pneumonia on pathologic analysis. These abscesses were surrounded by fibrosis, chronic inflammation, and a notable presence of eosinophils. The presence of cytotoxicity after the first injection of porcine umbilical cord tissue-derived cells (pUTC; *) may relate to nonspecific binding by IgM present in this animal (data not shown) as a result of this infection. (D) Animal 17307 demonstrated a decreased cytotoxic effect (**), which correlates with a decreased IgG titer (Fig. 2), after the second injection of pUTC possibly due to prednisolone treatment.