Figure 2.

Ilustration summarizing the cell culture methods used to generate retinal cells from embryonic stem cell cultures; the method name is in the upper left hand corner. Days in culture are indicated above the bars. Each culture ingredient is indicated by the same color words above or underneath the bars. Adherent cultures are solid bars while floating cultures are dots. CT, cholera toxin; RA, retinoic acid; LIF, leukemia inhibitory factor; FCS, fetal calf serum; DKK-1, Dickkopf WNT signaling pathway inhibitor 1; IGF-1, insulin-like growth factor 1; bFGF, basic fibroblast growth factor; ESCM, embryonic stem cell media; Retinal diff media, retinal differentiation media