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. 2013 Apr 12;154(6):2069–2080. doi: 10.1210/en.2012-2034

Figure 5.

Figure 5.

A, Effect of apelin (0.5nM to 50nM) on COB proliferation was assessed by using a BrdU incorporation assay. B, Effect of apelin on COB differentiation and mineralization was assessed by VK staining at days 28–35. The total colony area for VK staining was expressed as fold over basal. C, Effect of apelin on OB apoptosis was assessed by Cell Death Detection ELISA kit. D, Expression level of the adipocyte marker genes: eroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα). All expression data were obtained by RT-qPCR analysis of RNA isolated from 20-week-old femurs (n = 3 per group). E, Effect of apelin on adipogenesis. Oil Red O staining of lipid contents of the cell indicated the degree of adipogenesis. Total colony area stained positive for Oil Red O staining and total Oil Red O-positive colony number (CFU) were quantified as percent of basal level of control. The results of these assays were confirmed by repeating the experiment 3 times. All data are shown as mean ± SD. aP < .05; bP < .01.