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ERα and ERβ mRNA and protein levels in primary astrocyte cultures from 3 months and 24 months male rat cerebral cortex (n = 4 cultures, 4 brains per age group). Data are represented as percentage at 3 months (mean ± SEM). ***P < .001. A, ERα mRNA (RT-PCR) increased 3-fold by age; ERβ mRNA was unchanged. B, ERα protein (Western) increased 3-fold, with no change in ERβ. C, Representative Western blot showing single bands of ERα and ERβ. β-tub, β-tubulin loading control.
