Figure 4.

Gene expression in FSTL3-KO or FST288-only mouse liver or activin-treated HepG2 cells. A, Analysis of hepatic expression of genes involved with lipid uptake, de novo synthesis, lipolysis, insulin response, glucose uptake, or metabolism. Results are expressed relative to WT littermate gene expression. B, Gene expression in HepG2 cells after exposure to activin A (0.2nM or 2.0nM) for 48 hours to mimic chronic exposure in mouse liver. C, Gene expression in hepatocytes isolated from 12-week-old male C57BL6 mice treated with 2nM activin A for 48 hours. *, P < .05 (n = 4–5 for each gene).