Figure 2.

TNFα expression increases during constant light treatment. Total protein was isolated from dark-adapted albino retinas after 0 (Control), 16, and 36 h of constant light treatment. Immunoblotting for TNFα expression reveals a 28 kDa band, likely corresponding to the uncleaved form of TNFα. After 16 h of constant light, a 17 kDa band is detected that corresponds to the mature, secreted form of TNFα. Total protein was also isolated from dark-adapted albino dorsal retinas that were injected and electroporated with either the tnfα 5-mis control morpholino (MM) or the tnfα morpholino (MO) and placed in constant intense light for 36 h. While the tnfα 5-mis control retina had approximately the same amount of TNFα as the uninjected light-damaged retina (36 h), the tnfα morphant possessed dramatically less TNFα protein. β-actin expression was detected as a loading control.