Fig. 7.

Native murine perilipin 5 also forms high-density structures of a discrete size. Heart (A) and liver (B) samples from fasted 8–10 male C57/Bl6 mice were subjected to ultracentrifugation using extended discontinuous sucrose density gradients (0–60%) and examined using non-denaturing gradient gel electrophoresis as described in Fig. 2. Immunoblots were probed with anti-perilipin 5 antibodies. The approximate density at which staining was observed (d = 1.10–1.17 g/ml) and size of the bands (~ 575 kDa) was indistinguishable from those observed in the CHO model system. n = 5 animals, representative blots shown.