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13cRA mediates early phosphorylation of ERK1/2. (A) Western blot analysis of phospho-MAPK relative to total-MAPK after treatment with 25 μM 13cRA at indicated times. (B) Immunofluorescent microscopy time-course directed against phospo-MAPK after treatment to 25 μM 13cRA at indicated times. Anti-phospho ERK1/2 labeled with FITC (green) and nuclei stained with DAPI.
