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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: J Mol Endocrinol. 2013 Apr 23;50(3):361–374. doi: 10.1530/JME-12-0154

Figure 4.

Figure 4

Figure 4

13cRA mediates activation of Egr-1 expression. (A) Real-time quantitative PCR detection of Egr-1 transcripts plotted as a time course, reaching maximum expression at 20 min (***p<0.001 compared to control, n=3). (B) Western blot analysis of Egr-1 after treatment with 25 μM 13cRA at indicated times. (C) Immunofluorescent microscopy time-course directed against Egr-1 after treatment to 25 μM 13cRA at indicated times. Anti-Egr-1 labeled with FITC (green) and nuclei stained with DAPI.