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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: J Mol Endocrinol. 2013 Apr 23;50(3):361–374. doi: 10.1530/JME-12-0154

Figure 6.

Figure 6

Knockdown studies reveal Sp1 expression is essential for AT1R basal transcription. (A) Western blot displaying sufficient Sp1 silencing by siRNA targeted for Sp1. Gel separation shows doublet of protein bands characteristic with polyclonal antibody SC59-X. (B) Densitometric analysis of Sp1 bands from Western blot shown in panel A. Displayed is a knockdown by Sp1 siRNA of 90–95% relative to control. 13cRA has no effect on level of expression of Sp1. (C) AT1AR mRNA expression eliminated after Sp1 knockdown (-out). mRNA transcription characteristically reduced after 13cRA treatment. (D) Radio-labeled ligand binding assay showing elimination of binding of AngII to membrane receptors after Sp1 knockout. All data are expressed as mean ± SEM. “(−)Ctrl” and “(−)13cRA” indicate cells that are transfected with scrambled negative control siRNA. All 13cRA results shown are treated with 13cRA for 20 h.