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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: J Immunol. 2013 Jun 26;191(3):1476–1485. doi: 10.4049/jimmunol.1202514

Figure 3. IRF4 induces expression of ISGs in B-cells.

Figure 3

(A) Analysis of IRF4 protein induction following Dox stimulation in BJABiIRF4 cells. 2×106 cells were stimulated with increasing doses of Dox as indicated for 48. Lysates were prepared after stimulation and immunoblotted with anti-V5 and Actin antibodies.

(B), (C), (D), and (E) qRT-PCR analysis of IRF4 (B), ISG60 (C), Cig5 (D), and MxA (E) expression in Dox treated BJABiIRF4 cells. Cells were stimulated as previously described, total RNA was harvested and subjected to qRT-PCR. Samples were normalized to RPL32 and expressed as fold change with respect to untreated cells (value 1), marked with (#).