Fig. 5.

Differential effect of rpn10 mutant proteins. a Rpn10, rpn10^vwa and rpn10^uim were expressed in rpn11-1 rpn10Δ mutant (+P_GAL1 RPN11), and growth was determined on galactose and glucose medium, at both 23 and 37°C. ACY135, with or without an empty vector (second and third rows), showed a similar loss of viability on glucose. The effect of expressing an Rpn10 double mutation (rpn10^{vwa uim}) in rpn11-1 rpn10Δ was also determined. b The ability of the mutant rpn10 proteins to suppress the morphological defects of rpn11-1 rpn10Δ was examined by microscopy. Yeast cells were plated on glucose medium for 48 h and then examined with a Zeiss Axioplan microscope. A representative view of yeast cells, from both galactose and glucose medium (23 °C cultures) is shown