Fig. 6.

Suppression of rpn11-1 rpn10Δ by Rpn10 requires the VWA domain. Flag-epitope tagged derivatives of Rpn10 and rpn10 mutants were expressed from plasmids, and transformed into ACY135. Protein extracts were prepared from galactose a, and glucose b grown cultures. Equal amount of protein was resolved by SDS/PAGE and characterized by immunoblotting with antibodies against the proteins indicated (Extracts; left panels). An equal amount of protein extract was also incubated with Flag-agarose to immunoprecipitate the Flag-tagged proteins. The co-purified proteins were detected by immunoblotting, using antibodies against the proteasome subunits shown