Table 2.
Plasmids
| Plasmid | Description | Origin |
|---|---|---|
| pNOY285 | Derivative of pRS316-GAL-Rpn11 | M. Nomura |
| AEP105 | PCUP1–FLAG-Rpn10∷TRP1 | This study |
| AEP80 | PCUP1–FLAG-rpn10-vwa∷TRP1 | This study |
| AEP106 | PCUP1–FLAG-rpn10-uim∷TRP1 | This study |
| AEP107 | PCUP1–FLAG-rpn10-vwa-uim∷TRP1 | This study |
| AEP101 | pGEX-GST–Rpn10 | This study |
| AEP102 | pGEX-GST–rpn10-uim | This study |
| AEP103 | pGEX-GST–rpn10-vwa | This study |
| NEP10 | PCUP1–FLAG-rpn11(1–280)∷TRP1 | This study |
| NEP11 | PCUP1–FLAG-rpn11(1–288)∷TRP1 | This study |
| NEP12 | PCUP1–FLAG-rpn11(1–294)∷TRP1 | This study |
| NEP13 | PCUP1–FLAG-rpn11(1–299)∷TRP1 | This study |
| NEP14 | PCUP1–FLAG-rpn11(1–303)∷TRP1 | This study |
| HEP56 | PCUP1–FLAG-Rpn11(1–306)∷TRP1 | This study |
| LEP385 | pUNI50-Rpn8 | D. Skowyra |
| LEP386 | pUNI50-Rpn9 | D. Skowyra |
| LEP387 | pUNI50-Rpn10 | D. Skowyra |
| LEP389 | pUNI50-Rpn12 | D. Skowyra |
| LEP521 | pET28-his6-Rpn10 | This study |
| LEP545 | pET28-His6-Rpn8 | This study |
| LEP601 | pET28-His6-Rpn11 | This study |
| LEP544 | pGEX-GST-Rpn8 | This study |
| LEP405 | GST-Rpn9 | D. Skowyra |
| LEP406 | GST-Rpn10 | D. Skowyra |
| LEP408 | GST-Rpn12 | D. Skowyra |