Table 2.
PCR primers and methods for six gene loci in Bathymodiolus mussels
| Locus | Product | Primers | Ref. 1 | Methods | Length |
|---|---|---|---|---|---|
|
COI |
Cytochrome-c-oxidase subunit-I |
COIG/H |
[37] |
2 |
570 |
|
SAHH |
S-adenosylhomocysteine hydrolase |
BatSAHHF/370R |
[25]3 |
Fast PCR |
~400 |
|
ANT |
Adenine nucleotide (ADP/ATP) translocase |
AntF/AntR1 and AntF/AntR2 |
[38] |
TD and fast PCR |
496 |
|
Cat |
Catchin |
CatF/Cat2R |
4 |
Fast PCR5 |
~500 |
|
Col-1 |
Collagen type XIV |
Col160F/Col619R |
6 |
Fast PCR |
~430 |
| EF1α | Elongation Factor 1α | BatEf1αF/BatEf1αR | [25] | Fast PCR | 540 |
1 References in Literature Cited.
2 PCR program: 95°C/10 min; 35 x [94°C/1 min, 55°C/1 min; 72°C/2 min], extension at 72°C/7 min.
3 New to this study: Based on [25], SAHH370R, 5'-CGYTCGAACTCGCAAYGTYAGTGG-3'.
4 New to this study: CatF, 5'-GAGYGTCTTTCMAAGATCATCTCC-3'/Cat2R, 5'-CATTTYCTGATGTTWCGCTGGAT-3'.
5 Touchdown and Fast PCR: Amplitaq Gold Fast PCR Master Mix, UP (Life Technologies Corp., Carlsbad, CA) and the protocol for the taq supplied by manufacturer of Veriti thermal cycler with an annealing temperature at 50°C (Life Technologies Corp., Carlsbad, CA).
6 New to this study: Based on [25], Col160F, 5'-GGTTCACGAYCGGAWGTTCCCC-3', Col619R, 5'-TCCCAGCCTTTTCTCGCCCA-3'.