Figure 2.

Interplay between AdnAB and Ku during DSB processing in mycobacteria. Ku and AdnAB compete for binding to DSB ends. If Ku binds the DNA ends first, access to AdnAB is limited, thus favoring repair by NHEJ. If AdnAB is allowed to engage the DNA ends, 5′ resection occurs to expose 3′ ssDNA tails that are used to assemble the RecA presynaptic filament, and repair of the DSB occurs by HR. If the 3′ ssDNA tails are trimmed, subsequent repair by NHEJ will result in deletions at the DSB site.