Table 1.

Comparison of ¹⁸k_LG, ¹⁸k_NUC, and ¹⁸k_NPO KIE values for 2′-O-transphosphorylation catalyzed by acid, base, and RNase A

Catalyst	18kLG	18kNUC	18kNPO
RNase A, pH 7	1.014(3)	0.994(2)	1.001(1)
RNase A (QM)	1.026	0.998	1.006
Acid, pH 0	1.005(4)	0.990(4)	0.991(1)
Base, pH 12 (7, 8)	1.037(2)	0.996(2)	0.999(1)
Base, pH 14 (8)	1.034(4)	0.984(3)	—
Base, pH 14 (QM)	1.046	0.973	1.002

SDs for measured KIEs are shown in parentheses (Methods).